Peptide Storage & Handling: Don't Be Cringe

Nothing makes Peptide Pepe more Physically Sick than seeing researchers mishandle their compounds. Proper storage is the difference between Based research and Clown World results.

The Golden Rules of Peptide Storage

Rule 1: Temperature Matters (Feels Good Man at -20°C)

• Lyophilized peptides: Store at -20°C or below
• Reconstituted peptides: Store at 2-8°C
• Working solutions: Keep on ice during experiments
• Room temperature: Only during active use (minutes, not hours)

Rule 2: Light Is the Enemy (Evil Pepe Energy)

• UV light degrades peptide bonds
• Store in amber vials or wrapped in foil
• Minimize light exposure during reconstitution
• Work in low-light conditions when possible

Rule 3: Moisture Is Not Your Fren

• Keep lyophilized peptides sealed with desiccant
• Use dry nitrogen gas to displace oxygen in vials
• Never open cold vials until they reach room temperature (prevents condensation)

Reconstitution Protocol: The Thinking Pepe Way

1. Allow vial to reach room temperature (15-20 minutes)
2. Use bacteriostatic water or sterile water
3. Add solvent slowly down the side of the vial
4. Gently swirl — NEVER shake (shaking = Pepega behavior)
5. Allow to dissolve completely (may take 5-10 minutes)
6. Aliquot into single-use portions to avoid freeze-thaw cycles

Common Mistakes: The Pepega Hall of Shame

• Shaking vials vigorously: Causes foaming and denaturation
• Repeated freeze-thaw cycles: Each cycle degrades ~5-10% of peptide
• Using tap water: Only a Normie would do this
• Storing at room temperature: Guaranteed degradation

Stability Chart: Know Your Peptides

LABORATORY RESEARCH USE ONLY: All storage protocols are for laboratory research compounds only.

— Peptide Pepe, Chief Research Officer 🐸🔬